To perform the assay of Furosemide.

 Experiment

Object:

To perform the assay of Furosemide.

Chemical Structure:

Chemical Name:

4-chloro-N-furfuryl-5-sulphamoyl anthranilic acid

Molecular Formula: C12H11CIN2OS

Mol. Wt.: 330.7

Furosemide contains not less than 98.5 per cent and not more than 101.0 per cent of

C12H11CIN2O2S, calculated on dried basis.

Theory:

Category: Diuretic.

Description: A white or almost white, crystalline powder.

Loss on drying: Not more than 0.5 per cent, determined on 1.0 g by drying in an oven at

105°C.

Dose:

 Orally, in oedema, 20 to 40 mg daily, in oliguvria, 250 mg 4 to 6 times daily;

by intramuscular or slow intravenous injection, 20 to 50 mg (rate not exceeding 4 mg per

minute).

Assay:

• Weigh accurately about 0.5 g, dissolve in 40 ml of dimethyl formamide.
• Titrate with 0.1 M sodium hydroxide using bromothymol blue solution as an indicator.
• Carry out a blank titration.

Factor:

 1 ml of 0.1 M sodium hydroxide is equivalent to 0.03307 g of C12H11CIN2O5S.

Storage: Store protected from light.

Uses:

 It is used as diuretic.

It's used to treat high blood pressure, and heart failure and oedema (a build up of fluid in the body).

Reference:

Dr. Abhishek Tiwari, Dr. Rajeev Kumar " A practical book of medicinal chemistry" Nirali Prakashan Pune 2019 pp 66