To Determine the total alkaloid Content.

 Experiment

                         

                                   

Object: 

To Determine the total alkaloid Content.

Reference: 

Khandelwal, K., R., 2003. Practical Pharmacognosy; NIrali Prakashan ; Tenth  Edition; 158.

Requirement: 

DMSO, HCl, bromocresol green, phosphate buffer, Atropine.

Theory: 

Alkaloids are a group of naturally occurring chemical compounds that mostly contain basic nitrogen atoms. This group also includes some related compounds with neutral and even weakly acidic properties. Some synthetic compounds of similar structure are also termed alkaloids. In addition to carbon, hydrogen and nitrogen, alkaloids may also contain oxygen, sulfur and, more rarely, other elements such as chlorine, bromine, and phosphorus. 

Procedure: 

• The plant extract (1mg) was dissolved in dimethyl sulphoxide (DMSO) 
• Add 1ml of 2 N HCl and filtered. 
• Transfer the solution to a separating funnel and add 5 ml of bromocresol green solution and 5 ml of phosphate buffer. 
• The mixture was shaken with 1, 2, 3 and 4 ml chloroform by vigorous shaking and collected in a 10-ml volumetric flask and diluted to the volume with chloroform. 
• A set of reference standard solutions of atropine (20, 40, 60, 80 and 100 μg/ml) were prepared in the same manner as described earlier. The absorbance for test and standard solutions were determined against the reagent blank at 470 nm with an UV/Visible spectrophotometer.
•  The total alkaloid content was expressed as mg of AE/g of extract.

Result:

The total alkaloid Content was found to be_______.